TABLE 1.
Year sample no. | Aphid species1 (BlastN best hit) | Barley cultivar or variety2 | NGS group |
2016 | |||
BaA1-16-1 | Rhopalosiphum padi | cv. Morex | pool-1 |
BaA1-16-2 | Rhopalosiphum maidis | OUU659 | pool-1 |
BaA1-16-3 | Rhopalosiphum padi | cv. Kikai Hadaka | pool-1 |
BaA1-16-4 | Sitobion avenae | cv. Kikai Hadaka | pool-1 |
BaA1-16-5 | Rhopalosiphum padi | cv. Golden Promise | pool-1 |
2017 | |||
BaA2-17-6 | Rhopalosiphum maidis | cv. Golden Promise | pool-2 |
BaA2-17-7 | Sitobion avenae | cv. Kikai Hadaka | pool-2 |
2018 | |||
BaA3-18-8 | Rhopalosiphum maidis | OUK327 | pool-3 |
BaA3-18-9 | Rhopalosiphum maidis | OUU094 | pool-3 |
BaA3-18-10 | Rhopalosiphum maidis | cv. Minori Mugi | pool-3 |
BaA3-18-11 | Rhopalosiphum maidis | cv. Minori Mugi | pool-3 |
BaA3-18-12 | Rhopalosiphum maidis | cv. Kobin Katagi | pool-3 |
BaA3-18-13 | Rhopalosiphum maidis | cv. Kobin Katagi | pool-3 |
BaA3-18-14 | Rhopalosiphum maidis | cv. Ryofu | pool-3 |
BaA3-18-15 | Rhopalosiphum maidis | cv. Golden Promise | pool-3 |
1The species of the aphid colonies were identified by sequencing the nucleotides of fragments of a mitochondrial gene cytochrome c oxidase 1 (COI) using RT-PCR. Rhopalosiphum padi (the bird cherry-oat aphid); R. maidis (the corn leaf aphid); Sitobion avenae (the English grain aphid). 2The five aphid samples (BaA3-18-10 to BaA3-18-14) were obtained from the barley plants grown in a different area of the IPSR field.