Figure 2.

Raw well water differentially affects leukocyte recruitment following fungal and bacterial in vivo stimulation. Following 7d raw well water consumption, birds were challenged in vivo via intra-abdominal route using zymosan (fungal) or heat killed Salmonella enterica serovar Typhimurium (HKST; bacterial). Abdominal lavages were performed at 0, 4, 12, and 48 h post intra-abdominal challenge. (A) Total leukocytes were counted using a hemocytometer and light microscopy. (B) The proportions of heterophils, monocyte/macrophages and lymphocytes were determined using Imaging flow cytometry along with conventional Wright Giemsa staining. Data represents mean ± SEM (n = 7). Differences were evaluated using two-way ANOVA and Sidak's multiple comparison test. *p < 0.05.