Table 3.
Primers and annealing temperatures used for mRNA transcript quantification in real time QPCR analysis of liver and ovarian tissues.
| Gene | Accession numbers | Primer | Sequence (5′-3′) | Annealing temperature (°C) |
|---|---|---|---|---|
| β-actin | AB039726 | Forward | CCTCCATTGTTGGCAGACC | 57 |
| Reverse | CCTCTCTTGCTTTGAGCCTC | |||
| GAPDH | KT985226.1 | Forward | TGATGCTGGTGCCCTGTATGTAGT | 57 |
| Reverse | TGTCCTGGTTGACTCCCATCACAA | |||
| Vtg | DQ641252 | Forward | GAAGTGCGCATGGTGGCTTGTATT | 55 |
| Reverse | AGCTGCCATATCAGGAGCAGTGAT | |||
| IGF-I | GU583648.1 | Forward | CAGGGGCATTGGTGTGA | 57.1 |
| Reverse | GCAGCGTGTCTACAAGC | |||
| ERα | AY055725.1 | Forward | GAGGAAGAGTAGCAGCACTG | 55 |
| Reverse | GGCTGTGTTTCTGTCGTGAG | |||
| ERβI | AF061269.1 | Forward | GGCAGGATGAGAACAAGTGG | 55 |
| Reverse | GTAAATCTCGGGTGGCTCTG | |||
| TRαI | AY973629.1 | Forward | AGCCTGCCATGCCAGCC | 55 |
| Reverse | CCTCCTGATCCTCGAAGACC | |||
| TRβ | AY973630.1 | Forward | GAGGAGCAGCAGAAGACGG | 55 |
| Reverse | GTTGCCTTGGGCGTTTGTGG | |||
| FSHR | HM347775.1 | Forward | CGTCCACAATCCTACCTTCG | 56 |
| Reverse | TGAGAAACGGTGATTAGCGG | |||
| Cyp19a1 (aromatase) | AB009336.1 | Forward | TTGTGCGGGTTTGGATCAATGGTG | 58 |
| Reverse | TTCCGATACACTGCAGACCCAGTT |
All primers were designed and validated using Primer3 online software (Whitehead Institute of Biomedical Research, Cambridge, MA, USA).