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. 2020 Apr 7;7:88. doi: 10.3389/fmed.2020.00088

Figure 3.

Figure 3

circHIPK3 acts as a miR-193a-5p sponge. (A) QPCR was performed to detect the expression of miR-30a, miR-193a-5p, and miR-124 in serum samples of patients with AP and healthy subjects. MAP, mild acute pancreatitis; SAP, severe acute pancreatitis. (B) qPCR was performed to determine the expression of miR-30a, miR-193a-5p, and miR-124 in AR42J cells after caerulein treatment. (C) qPCR was performed to determine the expression of miR-30a, miR-193a-5p, and miR-124 after shRNA circHIPK3 transfection in AR42J cells. (D) qPCR was performed to determine the expression of miR-193a-5p after miR-193a-5p inhibitor transfection in AR42J cells. (E) CCK8 assay was performed to measure the cell viability in AR42J cells after caerulein treatment or with shRNA transfection and miR-193a-5p inhibitor transfection. (F) PI staining was performed to determine the cell pyroptosis after caerulein treatment or with shRNA transfection and miR-193a-5p inhibitor transfection. (G) Amylase activity was measured by ELISA kit after caerulein treatment or with shRNA transfection and miR-193a-5p inhibitor transfection in AR42J cells. (H) Levels of inflammatory cytokines IL-1β, IL-6, IL-8, and TNF-α were measured by ELISA kits in culture medium after caerulein treatment or with shRNA transfection and miR-193a-5p inhibitor transfection. (I) The pyroptosis-related proteins caspase 1 and caspase 11 were analyzed by immunoblot after caerulein treatment or with shRNA transfection and miR-193a-5p inhibitor transfection in AR42J cells (left), and the bands were quantified (right). *p < 0.05.