Figure 4.

Effects of EHT1864 on carbachol-induced contractions of human detrusor tissues. Contractions of female (A) and male (B) tissues from the lateral urinary bladder wall were induced by cumulative concentrations of carbachol in an organ bath, 30 min after addition of EHT1864 (100 µM) or an equivalent amount of water (controls), which was used as solvent for EHT1864. The stock solution of EHT1864 had a concentration of 10 mM, so that 100 µl of water was added to organ bath chambers. Each chamber contained 10 ml Krebs-Henseleit solution, resulting in a concentration of 0.99% for solvent-related water. Shown are data from n=5 female and n=5 male patients, which are means ± SD in concentration response curves (^#p < 0.05 for control vs. EHT1864 by two-way ANOVA, and p values for whole groups in inserts from 1-way ANOVA), and E_max and pEC₅₀ values for single experiments (calculated by curve fitting) where calculation was possible (see text) in scatter plots (p value from paired Student’s t-test).