Figure 5.

Adipocyte-exposed macrophages produce more angiogenesis regulating proteins. THP-1 were differentiated with PMA and either cultured alone (control) or with primary human breast adipocytes for 8 h, then changed to fresh media for an additional 16 h before collecting conditioned media, which was assayed on Angiogenesis Array nitrocellulose membranes pre-spotted with capture antibodies for 55 angiogenesis related proteins (n = 3, ^*p < 0.05); statistical analyses were performed using standard deviation and the unpaired Student's t-test. (A) IL-8; (B) MMP9; (C) TIMP1; (D) TSP1; (E) PTX3; and, (F) Serpin-E1/PAI1 protein expression levels. Endothelial tube formation assay was performed with adipocyte-exposed macrophage supernatant (H) and control macrophage supernatant (G). HUVEC incubated in media conditioned with macrophages not exposed to adipocytes. (I) Table of parameters measured that significantly increased in the HUVEC tube formation assay when HUVEC were incubated in media conditioned with macrophages cultured alone (control) or those co-cultured with adipocytes [statistical analyses were performed using standard deviation and the 2-way ANOVA with factors for experiment (n = 4) and treatment (macrophages cultured alone vs. macrophages co-cultured with adipocytes)].