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. 2020 Apr 7;10:380. doi: 10.3389/fonc.2020.00380

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Copyright © 2020 Yang, Jiang, Yao, Wang, Sun, Qin and Zhao.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Generation of senescent fibroblasts, and the cell cycle and senescence-associated markers in LV-GALC HFF1/HFL1 fibroblasts cells. (A) Construction of the LV-GALC HFF1/HFL1 fibroblasts cells. (B) The expression of GALC in normal HFF1/HFL1 cells (NC), LV-NC HFF1/HFL1 fibroblasts, and LV-GALC HFF1/HFL1 fibroblast cells. (C) β-galactosidase staining of LV-NC HFF1/HFL1 fibroblasts and LV-GALC HFF1/HFL1 fibroblast cells. (D) Cell cycle analysis of NC, LV-NC HFF1/HFL1 fibroblasts, and LV-GALC HFF1/HFL1 fibroblast cells. (E,F) qRT-PCR analysis of hTERT, p16, p21, and p53 expression in NC, LV-NC HFF1/HFL1 fibroblasts, and LV-GALC HFF1/HFL1 fibroblast cells. (G) The expression of p53, p21, and p16 proteins in NC, LV-NC, and LV-GALC HFF1/HFL1 cells. (H) Quantitative analysis of the expression of p16, p53, and p21 proteins in NC, LV-NC, and LV-GALC HFF1/HFL1 cells.