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. 2020 Apr 7;10:380. doi: 10.3389/fonc.2020.00380

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Copyright © 2020 Yang, Jiang, Yao, Wang, Sun, Qin and Zhao.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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In vitro impact of senescent fibroblasts on CRC cells. (A) After co-culture with LV-GALC fibroblasts cells, the proportion of G0/G1 phase LoVo cells shown at 24 and 48 h (p < 0.05) and the proportion of G0/G1 phase RKO cells shown at 48 h (p < 0.05). (B,C) Migration ability in a Transwell migration assay for RKO, LoVo, HCT116, and HT29 cells co-cultured with LV-GALC fibroblast cells and control cells (p < 0.05). (D,E) Ki-67 staining of LoVo cells co-cultured with LV-GALC fibroblast cells (p < 0.05). p < 0.05 was considered statistically significant.