TABLE 2.

Reproducibility of the qddMSP assay.

Donor	Day 16 of expansion						Day 23 of expansion
	% Demethylation repeats			Average	±SD	%CV	% Demethylation repeats			Average	±SD	% CV
1	82	83	84	83.0	1.00	1.20	89	91	89	89.67	1.15	1.29
2	96	97	95	96.0	1.00	1.04	91	92	93	92.00	1.00	1.09
3	94	93	94	93.7	0.63	0.67	82	83	84	83.00	1.00	1.20
4	89	89	90	89.3	0.58	0.65	66	64	63	64.33	1.53	2.37
5	71	70	72	71.0	1.00	1.41	91	92	92	91.67	0.58	0.63
6	94	95	95	94.7	0.58	0.58	88	86	85	86.33	1.53	1.77

Replicate assays were done on two separate time point, and each assay represents the mean data for at least three repeats. SD is the standard deviation, and % CV is the percent coefficient of variation [(SD/average)×100].