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. 2020 Apr 7;11:357. doi: 10.3389/fpls.2020.00357

FIGURE 5.

FIGURE 5

Principle of activity-based protein profiling for target identification and competitive activity-based protein profiling for the screening of glycosidase inhibitors. The competition of ABP labeling of a glycosidase by agents interacting with its pocket (inhibitors, substrates) can be conveniently and sensitively assessed. there is no need for a pure enzyme, when visualizing labeled glycosidase using SDS-PAGE and fluorescence scanning.