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. 2020 Apr 7;11:383. doi: 10.3389/fpls.2020.00383

FIGURE 7.

FIGURE 7

Yeast two-hybrid protein interaction assays. (A) The growth of yeast cells harboring the GL3 fused to the GAL4 DNA-binding domain (BD-GL3) or the GAL4 DNA-binding domain alons (BD-empty), and TTG1(WT), TTG1(ttg1-23), or TTG1(ttg1-24) fused to the GAL4 activation domain (AD) are shown. The yeast cells are grown on the SD/-Trp/-Leu/-His/-Ade selection medium with 20 mM 3-aminotriazole (3-AT). For serial dilution analysis, yeast cells were collected and adjusted to OD600 = 1.0, and diluted to 100, 10–1, and 10–2. (B) Yeast two-hybrid β-galactosidase activity assay quantifying the interaction between GL3 and TTG1(WT), TTG1(ttg1-23) or TTG1(ttg1-24). Three independent biological replicates were performed for each combination. Error bars represent standard deviations from the three replicates. Bars marked with the same letter indicate values not significantly different; bars marked with different letters indicate values showing statistically significant differences (P < 0.05; ANOVA test).