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. 2020 Apr 7;11:602. doi: 10.3389/fimmu.2020.00602

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Copyright © 2020 Anderson, Do, Toh, Hoe, Reitsma, Mulholland and Licciardi.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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The effect of VitD₃ on TLR expression in PBMCs. 1 × 10⁶ PBMCs/mL were treated with VitD₃ (100 nmol/L) at the time of stimulation. PBMCs were stimulated with live RSV at an MOI = 1, WCA (1 μg/mL) or WCA-RSV (designated “co”) prior to phenotyping by flow cytometry. Cell populations were determined by positive staining for cell surface obtained from live single lymphocytes. (A) TLR2+, (B) TLR4+, (C) TLR7+, (D) CD14+TLR2+, (E) CD14+TLR4+, (F) CD14+TLR7+, (G) CD56+TLR2+, (H) CD56+TLR4+, (I) CD56+TLR7+. Open circles represent untreated PBMCs, whilst closed circles represent VitD₃ treated PBMCs. Data shown represents median ± IQR; n = 8 per group.