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. 2020 Apr 7;14:313. doi: 10.3389/fnins.2020.00313

TABLE 1.

List of tests (presented in order of testing) and measures incorporated into the unified behavioral scores, which behavioral trait they are probing and the direction of influence.

Test Measure Score contribution Influence
Direct social interaction (Age 4 weeks) - Exploration time Sociability Excluded
- Time following host +ve
- Time being followed +ve
- Time sniffing host +ve
- Time self-grooming −ve
- Time attacking host +ve
- Time being attacked +ve
- Time immobile −ve
Social odor discrimination (Age 8 weeks) - Increase in frequency of visits to novel social odor Sociability +ve
- Increase in time spent sniffing novel social odor +ve
Social propinquity (Age 9 – 12 weeks) - Latency to first share Sociability −ve
- Time vacant −ve
- Duration of double occupancy +ve
Elevated zero maze (Age 12 – 13 weeks) - Crosses into open Anxiety −ve
- Time in open −ve
- Latency to enter open +ve
Light/dark box (Age 18 – 20 weeks) - Crosses into light Anxiety −ve
- Time in light −ve
- Latency to enter light +ve
3-chamber test (Age 20 – 22 weeks) - Crosses into empty Sociability −ve
- Crosses into occupied +ve
- Time in empty −ve
- Time in occupied +ve
- Latency to enter occupied −ve
- Mean time in occupied +ve
Fecal corticosterone metabolites (FCMs) (Age 22 weeks) - Change in FCM levels under stressed conditions Anxiety/Stress +ve