Table 4.

Measured T-tau concentration and relative percentages for each plasma sample through multiple freeze/thaw cycles

	Cycle 0		Cycle 1		Cycle 2		Cycle 3		Cycle 4		Cycle 5		Averaged relative %	cv
	concentration	relative %	concentration	relative %	concentration	relative %	concentration	relative %	concentration	relative %	concentration	relative %
Sample 1	16.68	100.00	16.28	97.60	17.05	102.22	16.19	97.06	16.00	95.92	15.81	94.78	97.52	2.8
Sample 2	18.17	100.00	18.93	104.18	17.02	93.67	18.59	102.31	16.65	91.63	16.93	93.12	96.98	5.5
Sample 3	31.71	100.00	31.47	99.24	30.68	96.75	31.38	98.96	30.28	95.49	29.32	92.46	96.58	2.9
Sample 4	29.29	100.00	30.43	103.89	29.42	100.44	29.11	99.39	28.58	97.58	27.37	93.44	98.95	3.5
Sample 5	23.93	100.00	23.41	97.83	23.52	98.29	23.44	97.95	23.54	98.37	23.98	100.21	98.53	1.1
Sample 6	29.47	100.00	28.66	97.25	27.68	93.93	28.94	98.20	29.51	100.14	29.31	99.46	97.80	2.4
Sample 7	32.88	100.00	31.47	95.71	32.02	97.39	33.36	101.46	33.63	102.28	31.69	96.38	98.64	2.8
Sample 8	39.74	100.00	39.69	99.87	39.67	99.82	39.61	99.67	37.79	95.09	37.89	95.34	97.96	2.4
Mean ± SD		100.0±0.00		99.44±3.10		97.81±3.02		99.38±1.77		97.06±3.29		95.65±2.88	−	−

T-tau concentrations are presented as pg/mL. SD, standard deviation; CV, coefficient of variation.