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Mitochondrial fusion machinery is specifically involved in glucose starvation-induced autophagy. GFP-Atg8 plasmids were expressed in yeast strains accordingly listed from (A–F). Yeast cells were grown to the log-growth phase, then subjected to glucose starvation (SD-G) or nitrogen starvation (SD-N) for 4 h. Autophagic activity was detected by western blot using anti-GFP antibody.
