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. 2020 Mar 10;7:4. doi: 10.21037/sci.2020.02.02

Figure 4.

Figure 4

CD44 is expressed in hematopoietic cells including microglia/macrophages within ischemic areas. Immunohistochemistry at post-stroke day 7 (A-L) demonstrates that some CD44+ cells within ischemic areas express CD45 [CD44 (B, C, and D: green), CD45 (B, C, and E: red), and DAPI (B-E: blue)] (arrows) and other various microglial/macrophage markers, including Iba1 [CD44 (G, H, and I: green), Iba1 (G, H, and J: red), and DAPI (G-J: blue)] (arrows), CD86 [CD44 (L, M, and N: green), CD86 (L, M, and O: red), and DAPI (L-O: blue)] (arrows), and CD206 [CD44 (Q, R, and S: green), CD206 (Q, R, and T: red), and DAPI (Q-T: blue)] (arrows). Results displayed are representative of three replicates (N=3). Scale bars =100 µm (B, G, L, and Q) and 50 µm (C, D, E, H, I, J, M, N, O, R, S, and T).