FIGURE 3.

Granule cell precursor proliferation was increased in BTBR mice postnatally. (A,B) BrdU staining (green) of sagittal section of cerebellar vermis at P3. Dotted line in (A₁–A₃,B₁–B₃) delimit external granular layer (EGL) where proliferative granule cells originate. Nucleus was counterstained with DAPI (blue). (C) Quantification of the EGL thickness showing thicker EGL in BTBR mice at P3 (Student’s t-test; n = 6,5). (D) Quantification of BrdU positive cells per mm² in EGL of each group at P3 (Student’s t-test; n = 6,5). (E) Quantification of total BrdU positive cells in EGL of sagittal section at P3 (Student’s t-test; n = 6,5). (F) Quantification of the percentage of BrdU positive cells in EGL at P3 (Student’s t-test; n = 6,5). (G,H) Ki67 staining (red) of sagittal section of cerebellar vermis at P3 Nucleus was counterstained with DAPI (blue). White panels in (G,H) are magnified in (G₁,H₁). (I) Quantification of Ki67 positive cells per mm² in EGL of sagittal section at P3 (Student’s t-test; n = 6,5). (J,K) Ki67 staining (red) of sagittal section of cerebellar vermis at P7. Nucleus was counterstained with DAPI (blue). White panels in (J,K) are magnified in (J₁,K₁). (L) Quantification of Ki67 positive cells per mm² in EGL of sagittal section at P3 (Student’s t-test; n = 6,6). All data are displayed as mean ± SD. *P < 0.05, **P < 0.01, ***P < 0.001. Scale bar: (A,B,G,H,J,K) 200 μm; (A₁–A₃,B₁–B₃) 10 μm; (G₁,H₁,J₁,K₁) 20 μm.