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. 2020 Apr 7;8:231. doi: 10.3389/fcell.2020.00231

FIGURE 4.

FIGURE 4

Radial migration of granule neurons in cerebella was not altered in BTBR mice. (A,B) NeuN stained (red) granule cells showing mature neurons were all distributed in inner granular layer (IGL) both in WT and BTBR mice at P7 Nucleus was counterstained with DAPI (blue). (C) Quantification of EGL thickness in each group at P7. (Student’s t-test; n = 6,6). (D) Schema graph of sagittal cerebella illustrating the observed region (black panel) in figure (E–H). (E–F) S100β positive Bergman glia somas in Purkinje cell layer (PCL) at P7. (G,H) GFAP positive Bergmann glia fibers in molecular layer (ML) at P7. (I) Quantification of Bergman glia somas in PCL per mm (Student’s t-test; n = 6,6). (J) Quantification of Bergman glia fibers in ML per mm. (Student’s t-test; n = 6,6). (K–N) Nissl staining of middle sagittal cerebellar section in adult (P90) WT and BTBR mice. (L,N) Are Magnified images of black panels in (K,M). Dotted line in (L,N) indicate the boundary between ML and PCL. All data are displayed as mean ± SD. Scale bar: (A,B,K,M) 200 μm; (E–H,L,N) 10 μm.