FIGURE 7.

Trpc gene differential expression disturbed cerebellar development in BTBR mice at postnatal day 14. (A) Hierarchical clustering of differential expression genes in cerebella of C57BL/6J and BTBR mice at postnatal day 14. (B) Enriched top five GO pathway in biological process. The former significant pathway involved in cerebella development is highlighted in red frame. Number of differentially expressed genes in each pathway is list side to the bar. (C) Heat map depicting 6 significant genes identified from highlighted pathway in B with PPI by STRING (Supplementary Figure S2). Trpc6 is highlighted in red frame. (D) Validation of gene expression in control and BTBR mice by real time PCR. (E) Validation of Trpc3, Trcp4 and Camk4 genes expression in control and BTBR mice by real time PCR.