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. 2020 Mar;8(5):203. doi: 10.21037/atm.2020.01.34

Table 2. Bacterial species and strains used in this study and results of the multiplex PCR assay.

Strain No. Reference straina Multiplex PCR result for
nucb mecAc spyMd oprIe
1 MRSAf + +
2 MRSA + +
3 MRSA + +
4 MRSA + +
5 MRSA + +
6 MRSA + +
7 MRSA + +
8 MRSA + +
9 MSSA +
10 MSSA +
11 MSSA +
12 MSSA +
13 MSSA +
14 MSSA +
15 MSSA +
16 MSSA +
17 Staphylococcus epidermidis +
18 Staphylococcus epidermidis
19 Staphylococcus haemolyticus +
20 Staphylococcus haemolyticus +
21 Streptococcus pneumoniae
22 Streptococcus pneumoniae
23 Streptococcus pyogenes +
24 Streptococcus pyogenes +
25 Streptococcus pyogenes +
26 Streptococcus pyogenes +
27 Streptococcus pyogenes +
28 Streptococcus pyogenes +
29 Streptococcus pyogenes +
30 Pseudomonas aeruginosa +
31 Pseudomonas aeruginosa +
32 Pseudomonas aeruginosa +
33 Pseudomonas aeruginosa +
34 Pseudomonas aeruginosa +
35 Pseudomonas aeruginosa +
36 Pseudomonas aeruginosa +
37 Pseudomonas aeruginosa +
38 Pseudomonas aeruginosa +
39 Pseudomonas aeruginosa +
40 Human genome 1
41 Human genome 2
42 Human genome 3

a, clinical isolates obtained from the Microbiology Department of SCMC; b, to detect S. aureus; c, to identify methicillin-resistance; d, to detect S. pyogenes; e, to detect P. aeruginosa; f, only be confirmed when both nuc and mecA are positive; +: positive result; −: negative result. PCR, polymerase chain reaction; MSSA, methicillin sensitive Staphylococcus aureus; MRSA, methicillin resistant Staphylococcus aureus.