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. 2020 Mar;8(5):168. doi: 10.21037/atm.2020.02.21

Table 1. Sequences of primers for quantitative real-time PCR.

Gene name Forward primer sequence (5'-3') Reverse primer sequence (5'-3')
Rat
   NLRP3 TCTGTTCATTGGCTGCGGAT TAGCCGCAAAGAACTCCTGG
   IL-1β TTGAGTCTGCACAGTTCCCC TCCTGGGGAAGGCATTAGGA
   IL-18 CAGAAGCTGGGGTTGGTGAA CCCATGTCTCCAAGGGCATT
   Caspase 1 GGAGCTTCAGTCAGGTCCAT CTTGAGGGAACCACTCGGTC
   aSMA GGAGATGGCGTGACTCACAA CGCTCAGCAGTAGTCACGAA
   Collagen I CCCAGCGGTGGTTATGACTT TCGATCCAGTACTCTCCGCT
   TGFβ AGGGCTACCATGCCAACTTC CCACGTAGTAGACGATGGGC
   CTGF CTTCCCGAGAAGGGTCAAGC TCTTCCAGTCGGTAGGCAGC
   Fibronectin GGATCCCCTCCCAGAGAAGT GGGTGTGGAAGGGTAACCAG
   TIMP1 GCCTCTGGCATCCTCTTGTT AGCGTCGAATCCTTTGAGCA
   Bax TGGCGATGAACTGGACAACA TAGAAAAGGGCAACCACCCG
   Caspase 3 GCTGGACTGCGGTATTGAGA TAGTAACCGGGTGCGGTAGA
   β-actin GAACCCTAAGGCCAACCGTG AACCGCTCATTGCCGATAGT