Figure 1.

The protective effect of EGCG on H₂O₂-induced decreased cell viability in HUVECs. (A) HUVECs were incubated with increasing concentrations of H₂O₂ (100–1,000 µmol/L). n=3. **, P<0.01, ***, P<0.001 vs. control group. (B) Effect of different concentration of EGCG on the viability of HUVECs. (C) Effect of EGCG pretreatment on the viability of HUVECs treated with H₂O₂. HUVECs were exposed to various concentrations of EGCG (1, 5, 10 µmol/L) for 24 h. Then, cells were treated with H₂O₂ (200 µmol/L) for 4 h and cell viability was tested by MTS assay. n=3. **, P<0.01 vs. H₂O₂ alone treatment group. (D) Representative staining with TUNEL and PI. (E) The number of TUNEL-positive nuclei was expressed as a percentage of the total nuclei detected. n=5. ^###, P<0.001 vs. control group, ***, P<0.001 vs. H₂O₂ alone group. Data are shown as the mean ± SEM.