Fig 5.

Pneumococci adhere to DEPs in vitro. A-C, Following coincubation of D39 with DEPs, pneumococci (highlighted in purple) can be seen in close proximity to the particulate by scanning electron microscopy. Original images are shown in Fig E6. D-G, Flow cytometry plots for D39-GFP without slow-speed centrifugation step (D), coincubated DEPs and D39-GFP after slow-speed centrifugation (E), D39-GFP only after slow-speed centrifugation (F), and DEPs alone (G) are shown. The percentages of unbound and FSC-high GFP events in (D) and (E) are displayed in (H) and (I), respectively.