Figure 5.

Influence of PDEδ targeting modalities on SRE‐mediated gene expression and the level of lipid metabolites. A) HeLa cells were transfected with a firefly luciferase (Fluc) construct under the control of SRE and a plasmid for constitutive Renilla luciferase (Rluc) expression. Cells were treated with the compounds for 24 h prior to determination of Fluc and Rluc activity. Where indicated, cells were co‐treated with 2.5 μm 25‐hydroxycholesterol and 25 μm cholesterol. Data are mean values±SD (n=3). B) HeLa cells were treated for 24 h with 1 μm deltasonamide 1. Metabolites were quantified using mass spectrometry. Absolut values for significant quantification of metabolites are shown in the table. C) Obtained values were normalized to the values of DMSO‐treated cells. Data are mean values±SD (n=3). * p ≤0.05, ** p ≤0.01, *** p ≤0.001, MVA=mevalonic acid, IPPP=isopropyl pyrophosphate, HMG‐CoA=3‐hydroxy‐3‐methylglutaryl‐CoA, DMAP=dimethylallyl pyrophosphate, GPP=geranyl pyrophosphate, FPP=farnesyl pyrophosphate.