Figure 3.

CD8⁺IFNγ⁺ cellular immune responses following vaccination of rats with an adenoviral vectored vaccine encoding the nonstructural proteins of RHV (ChAd‐NS). Four male SD rats were vaccinated intramuscularly with 10⁸ i.u. per dose of an adenoviral (ChAdOx1) vectored vaccine encoding the nonstructural proteins of RHV shown in (A). Truncated shark invariant chain was used as a genetic adjuvant. PBMCs were isolated from blood drawn weekly from vaccinated rats and stimulated with four pools of overlapping peptides representing the nonstructural regions of RHV. (B) The time course of CD8⁺IFNγ⁺ responses for 4 weeks postvaccination, as measured using flow cytometry; plotted are pooled responses against all four peptide pools for each rat. Mock‐vaccinated rats are shown with hatched lines. (C‐E) Immune responses by flow cytometry 4 weeks postvaccination against the four individual peptide pools as shown for PBMCs, splenocytes, and liver‐infiltrating lymphocytes, respectively, with mock‐vaccinated rats shown as unfilled symbols. Abbreviations: CMV, cytomegalovirus; SIi, shark invariant chain.