Figure 7.

Protective efficacy of ChAd‐S alone and in combination with ChAd‐NS. Male SD rats (n = 6/group) were vaccinated by intramuscular injection with either ChAd‐S, ChAd‐NS, or premixed ChAd‐S and ChAd‐NS at a dose of 10⁸ i.u. per distinct vaccine. Four weeks following vaccination, rats were challenged by intravenous injection into the tail vein of 10⁵ vp RHV. Blood viremia was traced weekly postinfection by real‐time quantitative PCR against a standard curve of RHV RNA for (A) ChAd‐S‐vaccinated and (B) ChAd‐S + ChAd‐NS–vaccinated rats. (C) Viremia at EOT. Blood cellular immune responses were compared by flow cytometry for single‐vaccine versus combination groups for (D) CD8⁺IFNγ⁺ responses to structural antigens and (E) CD4⁺IFNγ⁺ and (F) CD8⁺IFNγ⁺ responses to nonstructural antigens at 28 days postchallenge.