Table 1.

Comparison of the aldehyde substrate scope of OXC_Me and HACL_Hs.^[a]

Aldehyde	R	Product name	OXCMe [c]	HACLHs [c]
2 a	H	glycolyl‐CoA	100	11
2 b	Me	lactyl‐CoA	74	100
2 c	CH2Me	2‐hydroxybutyryl‐CoA	5	100
2 d	CH2OH	glyceryl‐CoA	1	100
2 e	CHOHCH2OH	erythronyl‐CoA	n.d.[b]	n.d.[b]
2 f	COOH	tartronyl‐CoA	n.d.[b]	n.d.[b]
2 g	(CH2)2COOH	2‐hydroxyglutaryl‐CoA	1	100
2 h	Ph	mandelyl‐CoA	100	3
2 i	CH2Ph	3‐phenyllactyl‐CoA	22	100

[a] The reaction contained 2 a–2 i (10 mm), formyl‐CoA (1 mm), OXC_Me or HACL_Hs (5 μm). Products were analyzed by LC‐MS after 1 h reaction time. [b] Product not detected. [c] Relative activity in %. Relative activity refers to the comparison of OXC_Me and HACL_Hs for each aldehyde substrate.