Table 1.
Spectral properties of the dyes and their m/z ratios.
|
Dye (m/z) |
Absorption λ max [nm] (ϵ, m −1 cm−1) |
Emission λ max [nm] (Φ fl)[a] |
Solvent |
Fluor. lifetime τ [ns] |
|---|---|---|---|---|
|
APTS [b] (151) |
424 (20 600) |
500 (0.95) |
aq. PBS |
– |
|
APTS‐G6 [c] |
455 (17 000) |
511 |
H2O |
– |
|
6‐H (144) |
471 |
544 (0.88) |
H2O |
5.9 |
|
7‐H |
477 (22 400) |
535 (0.96) |
MeOH |
5.6 |
|
7‐Me |
493 (23 000) |
549 (0.97) |
MeOH |
5.9 |
|
9 |
502 |
563 (0.85) |
H2O |
3.6 |
|
13 |
502 (23 400) 509 (19 500) |
550 (0.88) 563 (0.67) |
MeOH H2O |
6.3 6.4 |
|
15 |
486 (21 000) |
534 (0.80)[d,e] |
MeOH |
4.9 |
|
16 (137) |
477 (19 600) |
542 (0.92) |
TEAB[f,g] |
5.8 |
[a] Absolute values of the fluorescence quantum yields (if not stated otherwise). [b] Data from Ref. 6b, abs. measured in H2O, emission measured in aq. phosphate buffer at pH 7.4. [c] Conjugate with maltohexaose (G6), data from Ref. 5b. [e] Excitation at 375 nm. [e] Rhodamine 6G as a reference dye with Φ fl=0.9. [f] Aq. Et3N*H2CO3, pH 8–8.5. [g] Fluorescein as a reference dye with Φ fl=0.9 in 0.1 m NaOH.