Composition	Volume	Final concentration
IMDM	117.25 ml
Ham's F‐12 nutrient mixture (F12; see recipe)	117.25 ml
PVA (5%; see recipe)	50 µl	10 mg ml−1
lipids (100×; see recipe)	250 µl	0.1%
ITS‐X (100×; see recipe)	5 ml	2%
αMTG (1.3%; see recipe)	750 µl	40 µl L−1
AA2P (5 mg ml−1; see recipe)	3.2 ml	64 mg L−1
Glutamax (200 mM)	2.5 ml	2 mM
non‐essential amino acid (NEAA) solution (100×)	2.5 ml	1%
Pen/Strep (5000 U ml−1)	1.25 ml	0.5%