Table 1.

Key findings related to NPC composition and transport function in aging. ↓ indicates a decrease in protein abundance, while – indicates no change in protein abundance was detected. The font colors indicates the location of the protein in the NPC, see Fig. 1: central FG‐Nups (green), inner ring (purple), outer ring (dark red), nuclear basket (gray), and RNA export platform (pink).

Study	Model organism	Nup protein abundances		Nup transcripts abundances	Transport phenotype
D'Angelo et al., 2009 4	Caenorhabditis elegans rat brain (24–28 month)	Nup93↓ FG‐Nups↓ Nup107‐	Nup93, Nup153 carbonylated	Downregulation of scaffold Nups in differentiated cells	Increased passive permeability of the NE of isolated nuclei and intranuclear tubulin bIII in vivo
Kim et al., 2010 12	Senescent human fibroblast (> 66 doublings)	Nup88↓ Nup107↓ Nup155↓	Nup50↓	Decreased transcript levels of Nups, but also importin α/ß and Ran system	Fewer NPCs, decreased nucleocytoplasmic transport, unresponsive to cell stimuli
Lord et al., 2015 13	Saccharomyces cerevisiae, replicative aging (6–9 divisions)	Nup100– Nup53– Nup116↓	Nsp1↓	Stable transcript levels of Nup100, nup53, Nup116 and Nsp1	Decreased nuclear accumulation of different NLS‐GFP reporters at steady state
Rempel et al., 2019 11	Saccharomyces cerevisiae replicative aging (multiple time points)	Nup100↓ Nup133– Nup116↓ Nup2↓ Nup120 ‐			Increased nuclear accumulation of NLS‐GFP reporters and Rcc1 at steady state and decreases exclusion of NES‐GFP reporters, decreased transport dynamics of Msn2 shuttling
Janssens et al., 2015 2	Saccharomyces cerevisiae replicative aging (12 time points)	Nup116↓↓ Nup2↓↓ Nsp1↓↓ Nup100↓ Nup60↓	Nup1↓ Mlp1↓ Gle2↓ 15 others‐	Stable transcript levels for all Nups