Skip to main content
. 2019 Dec 31;226(2):460–475. doi: 10.1111/nph.16362

Figure 6.

Figure 6

Transcripts of cell wall‐modifying enzymes in RIPENING INHIBITOR (RIN)‐deficient and wild‐type (WT) fruits measured by quantitative reverse transcription (qRT)‐PCR. (a) Genes whose transcripts are higher in RIN‐deficient fruits compared to WT fruits during at least one ripening stage. (b) Genes whose transcripts are lower in RIN‐deficient fruits compared to WT. Expression of genes was measured in fruits selected at the breaker (B), breaker + 5 (B + 5) and breaker + 10 (B + 10) stages. Transcript levels were determined by qRT‐PCR, relative to the expression of the tomato ACTIN gene internal control, expressed as 2‐ΔΔCt (Livak & Schmittgen, 2001): polygalacturonase (PG), expansin 1 (EXP1), cellulase (CEL2), pectinesterase (PE1/PME1.9, PME2.1), pectate lyase (PL), α‐mannosidase (MAN1), endo‐1,4‐β‐mannanase (MAN4a), β‐d‐xylosidase (XYL1), β‐mannosidase (Mside1), endo‐1,4‐β‐mannosidase 7 (Mside7), tomato β‐galactosidase (TBG4), xyloglucan endotransglucosylase/hydrolase (XTH5 and XTH8). The error bars represent mean ± SD, the lowercase letters indicate significant difference at P = 0.05.