Figure 1.

Isolation and identification of the TrCNX1 gene in Tradescantia. (a) PCR amplification of TrCNX1 from genomic DNA. (b) PCR amplification of TrCNX1 ORF from cDNA of Tradescantia. Target-specific amplicons indicated by an arrow. (c) Structural analysis of the TrCNX1 gene. In TrCNX1, the start (ATG) and stop (TGA) codons are shown in the gene coding region. The numbers above the TrCNX1 gene represent the DNA base pair (bp). Corresponding positions of introns (I)/exons (E) are indicated by arrows with the bp position. Numbers of introns (I1–I5) and exons (E1–E6) are denoted by white and gray boxes, respectively. (d) General structural features of the TrCNX1 protein. The putative conserved domains are shown according to previous reports [27]. The gray box indicates the conserved central domain of the endoplasmic reticulum (ER) lumen and those numbered 1 and 2 represent Ca²⁺-binding regions (motif 1) and oligosaccharide-binding domains (motif 2), respectively. The dark gray box represents the transmembrane (TM) domain in the cytosol. The size of each domain is denoted in closed brackets with amino acid (aa) residue lengths.