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. 2020 Mar 6;9(3):335. doi: 10.3390/plants9030335

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© 2020 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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A9 enhanced the accumulation of the CRY1 protein in transgenic tobacco. Detection of CRY1 antibody-reacting proteins in total protein samples from imbibed seeds and seedlings grown under darkness. Results for 10-day-old seedlings illustrated with samples from a representative sibling pair of nontransgenic (NT) and transgenic (T) (35S:A9) lines. Results for seeds imbibed for 24 h depicted for representative pairs of NT and T (DS10:A9 or DS10:A9M3) lines. Equal loading of total protein per lane was verified with antibodies against actin (ACT).