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. 2020 Mar 6;9(3):335. doi: 10.3390/plants9030335

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© 2020 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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A9 loss-of-function effect on seed deterioration in the DS10:A9M1 homozygous lines. (A) Results of Basal Thermotolerance Assay (BTA) assays performed with seeds from three different DS10:A9M1 lines compared with the sibling NT lines. Average germination percentages at different times after BTA (for a total of three independent experiments per line) are shown. Representative photographs of DS10:A9M1 (B) and sibling NT seed survival (C) 15 days after BTA in both cases. Scale bars: 1 cm. The observed seed longevity reduction for DS10:A9M1 was moderate and statistically significant, as expected from our precedent analyses of heterozygous lines (see Figure 2A in [7]). However, the observed reduction was lower than what previously reported for homozygous DS10:A9M3 seeds (see Figure 3 in [7]). Furthermore, statistics confirmed that the effects of DS10:A9M1 and DS10:A9M3 on longevity in homozygous seeds were highly significantly different (for statistics, see Table S7).