Figure 7.

Similar A9 loss-of-function effect on seedling greening in the DS10:A9M1 (A9M1) and DS10:A9M3 (A9M3) homozygous lines. Seeds from the different transgenic (A9M1 or A9M3) and the corresponding sibling NT lines were germinated and kept under darkness for 4 days. After dark germination, seedlings were transferred to continuous white-light conditions for 6 h or 16 h, as indicated, before pigment extraction and quantification. (A) Comparison of greening reduction using total chlorophyll content. (B) Comparison of greening reduction using total carotenoid content. Average photopigment content was determined and depicted as in Figure 2. Percent pigment content with respect to NT is provided in the panels to the right in each case. Statistical analyses did not find significant differences between pigment content reduction observed in the A9M1 and A9M3 seedlings after either 6 h or 16 h illumination (see Table S7). Data from at least three experiments, performed with two different pairs of sibling lines. Error bars denote the SEM.