Table 2.
Number of markers obtained from random amplified polymorphic DNA (RAPD) analysis of Secale cereale seeds (stored or stored-imbibed) and seedlings produced from them. Seeds were stored at 35 °C, 15% wc under two different storage atmospheres: air or vacuum. DNA was isolated from two replicates of 50 seeds or of 10 seedlings, per storage condition.
| Storage Atmosphere | Number of Polymorphic Markers | |||||||
|---|---|---|---|---|---|---|---|---|
| Stored Seeds | Stored-Imbibed Seeds | Seedlings | ||||||
| Storage Time (Days) | Storage Time (Days) | Seed Storage Time (Days) | ||||||
| 13 (P75) |
29 (P20) |
36 (P0) | 13 (P75) |
29 (P20) |
36 (P0) | 13 (P75) |
29 (P20) |
|
| Air | 3 | 4 | 5 | 0 | 1 | 1 | 4 | 5 |
| Vacuum | 0 | 2 | 3 | 2 | 1 | 1 | 4 | 5 |
| Total | 134 | 134 | 134 | 134 | 134 | 134 | 104 | 104 |