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. 2020 Mar 11;9(3):353. doi: 10.3390/plants9030353

Figure 2.

Figure 2

Subcellular localization of pyruvate kinase isoforms. cPK2, cPK4, cPK5-YFP fusion proteins (yellow) and TPT-GFP fusion protein (cyan) were expressed under the control of the cauliflower mosaic virus promoter (Pro35S), while free mCherry fluorescence protein was expressed under the control of the ubiquitin promoter (ProUbi). All PK-YFP fusion proteins were co-localized with free mCherry in cytosolic plasma strands (white arrows), whereby TPT-GFP fusion protein was co-localized with chlorophyll A fluorescence (green) as indicated by the white asterisks. Constructs were transiently expressed in Nicotiana benthamiana leaves. Scale bars = 50 µm.