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Programmed gradient elution of mobile phase for liquid chromatography/mass spectroscopy (LC/MS) analysis.
| Time (min) | Ratio of A (%) | Ratio of B (%) |
|---|---|---|
| 0–3.0 | 30 | 70 |
| 3.0–4.0 | 30–10 | 70–90 |
| 4.0–5.0 | 10–2 | 90–98 |
| 5.0–12.0 | 2 | 98 |
| 12.0–12.1 | 2–30 | 98–70 |
| 12.1–15.0 | 30 | 70 |
