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. 2020 Mar 4;9(3):323. doi: 10.3390/plants9030323

Figure 5.

Figure 5

Analysis of RNA secondary structure of an antisense tRNA between psbC and psbZ. The trnS-UGA gene encoding for the tRNA-Ser(UGA) is localized on the opposite strand of the psbD/C/Z operon, so that an antisense copy of the tRNA is included in the operon between psbC and psbZ. (A) Normalized DMS reactivities at the antisense tRNA sequence of in vivo-and in vitro-folded samples. A value close to 0 indicates a low amount of structure, a value close to 1 a high amount of structure. The dark grey bars represent the more reliable probing of adenosines and cytidines (compared to Figure 1B,C), light grey is the less reliable probing of guanosines and uridines. (B) The secondary structure of tRNA-Ser(UGA) is predicted by tRNAscan-SE v. 2.0 [39,40]. (C) Predicted mRNA secondary structure of the antisense tRNA sequence using DMS reactivities of the in vivo samples as constrains. The color code indicates the normalized DMS reactivities at adenosines and cytidines.