Table 3.
The primers used for detection of rs217727 and rs2107425 polymorphisms in lncRNA H19 gene.
| Gene polymorphisms | Chr:position | Product size | Primers | Sequence (5′ to 3′) |
|---|---|---|---|---|
| rs217727 | Chr11:1995678 | Outer primers: 397 bp | FO | ATGACTCAGGAATCGGCTCTGGAAGGTG |
| RO | GGGGAAACAGAGTCGTGGAGGCTTTGA | |||
| C allele: 248 bp | FI | TCATCTTCATGGCCACCCCCTGCTGT | ||
| T allele: 200 bp | RI | ATATGGTGGCTGGTGGTCAACCGTACG | ||
|
| ||||
| rs2107425 | Chr11:1999845 | Outer primers: 266 bp | FO | ACTTGAGTCCCAGGCCATGACACTGAAG |
| RO | CGGAATTGGTTGTAGTTGTGGAATCGGA | |||
| T allele: 195 bp | FI | CGACCTGAAGATCTGGTGCGGCTACT | ||
| C allele: 124 bp | RI | GGGTCATCTGGGAATAGGACACTCCTG | ||