Figure 2.

Cytochrome c reductase activity of yeast Erv1. (A) Time courses of cytochrome c (10 μm ScCytc or hhCytc) reduction by 1 mm DTT in the presence or absence of 1 μm Erv1. The reaction was initiated using stopped‐flow fast mixing technique and followed by measuring the absorbance change at 550 nm. (B) Cytochrome c (hhCytc in grey, ScCytc in black) concentration dependence of the Erv1 catalysed reaction. The initial rates (after subtraction of nonenzymatic background rate) were fitted with the Michaelis–Menten equation (solid lines). The kinetic parameters were summarised in Table 1. The error bars represent standard error of mean (SEM), n = 3.