Figure 3.

CCR4 is replenished at the cell surface by de novo protein synthesis following ligand‐induced internalization. (A) Cell surface CCR4 replenishment in CHO‐CCR4 cells following stimulation with 100 nM CCL17 or 100 nM CCL22 for 30 min at 37°C. Data are presented as the mean ± sem of 4 independent experiments. Panels (B)–(E) show replenishment of CCR4 at the cell surface of CHO‐CCR4 cells (B and D) and Th2 cells (C and E) following receptor internalization induced by treatment with 100 nM CCL22 (B and C) or 100 nM CCL17 (D and E). Cells were incubated in media with or without 10 µg/ml cycloheximide (CHX) during the 6‐h replenishment period. Data are presented as the mean ± sem of 3 independent experiments and was analyzed by one‐way ANOVA with Bonferroni's multiple comparisons