Figure 4.

CCR4 undergoes constitutive internalization in Hut78 and CHO‐CCR4 cells. (A) Constitutive CCR4 loss from the surface of Hut78 and CHO‐CCR4 cells over a 6‐h time course. (B) CCR4 degradation over the same period in whole cell lysates generated from CHO‐CCR4 cells incubated in simple media in the absence of chemokine. (C) Densitometry analysis of the data shown in (B). Data are presented as the mean ± sem of 5 independent experiments that were analyzed by one‐way repeated measures ANOVA (A) and 2‐way repeated measures ANOVA (C) with Bonferroni's multiple comparisons