Figure 4.

PRF reduces osteoclast differentiation induced by RANKL, M‐CSF and TGF‐β. Bone marrow cells were grown in the presence of 50% soluble extracts of PRF membranes to modify osteoclastogenesis induced by RANKL, M‐CSF and TGF‐β. (A) Representative images of TRAP+ multinucleated osteoclasts in the control group (M‐CSF) and in the absence or presence of PRF. (B) Mean number ± SD of TRAP+ osteoclasts in absence or presence of PRF. (C) Data represent the x‐fold changes in gene expression compared to an M‐CSF control. N = 4‐6. Statistical analysis was based on Mann‐Whitney U test. Significant changes are indicated by *P < 0.05 and **P < 0.01