Figure 1.

Schematic depiction of the centrifugation‐based filtration protocol. Briefly, the protocol includes a centrifugation step for the isolation of the fibril (pellet) from soluble α‐syn (monomers and oligomers), a filtration step (through 100 kDa MWCO) for the separation of the monomers from the oligomers, and a final step for the recovery of the oligomers from the spin filters. A depiction of the sodium dodecyl sulphate–polyacrylamide gel electrophoresis (SDS‐PAGE) analysis of the samples at each step is shown in the middle. Quantification of band intensity should allow for a quick assessment of the distribution of coexisting α‐syn species