Figure 2.

NK1.1⁺ ILC1s Accounted for a Higher Percentage of Lymphocytes in the Skin of Fetal/Neonatal Mice than of Adult Mice

(A) Flow cytometric detection of CD3⁻NK1.1⁺ cells in gated CD45⁺ lymphocytes of the skin of late fetal (E18) and neonatal (day1–4) CCR10^+/EGFP mice. Data are representative of three experiments with at least five mice for each experiment.

(B) Flow cytometric analysis of CCR10(EGFP) expression on gated CD3⁻NK1.1⁺CD45.2⁺ cells of the skin, intestine, spleen, and liver of fetal/neonatal CCR10^+/EGFP mice. Data are representative of 3–5 experiments.

(C) Flow cytometric detection of CD3⁻NK1.1⁺ cells in gated CD45.2⁺ lymphocytes of the skin of 1- and 3-month-old mice.

(D) Average percentages of CD3⁻NK1.1⁺ cells of skin CD45⁺ lymphocytes based on the analysis in (C). One dot represents one mouse.

(E) Flow cytometric analysis of CCR10(EGFP) expression on gated CD3⁻NK1.1⁺CD45.2⁺ cells of the skin, intestine, spleen, sLN, mesenteric LN (mLN), and liver of adult CCR10^+/EGFP mice. Representative of at least 10 experiments for skin, sLN, mesenteric LN (mLN), spleen, and liver.

(F–I) Flow cytometric analysis of the expression of CCR10 and indicated molecules in gated CD3⁻NK1.1⁺CD45.2⁺ cells of the skin of newborn CCR10^+/EGFP mice. Data are representative of at least two experiments.

(J) Flow cytometric analysis of the CD127 and CD11b expression on gated CCR10⁺ and CCR10^- CD3⁻NK1.1⁺CD45.2⁺ cells of sLNs of adult CCR10^+/EGFP mice. Data are representative of at least 10 experiments.

(K) Comparison of percentages and numbers of the CCR10⁺ and CD127⁺CD11b⁻ CD3⁻NK1.1⁺ cells in the skin, sLN, and thymus of adult CCR10^+/EGFP mice fed with FTY720 (FTY) or not (Ctrl). One dot represents one mouse.

(L) Flow cytometric analysis of gated EGFP⁺ CD3^- CD45⁺ cells of newborn and adult skin and adult sLNs of CCR10^+/EGFP mice for their expression of NK1.1.

Data are representative of at least three experiments. NS, not significant, ∗∗p < 0.01, ∗∗∗p < 0.001 as determined by two-tailed Student's t test.