Figure 3.

Different Thymic Environments and Progenitor Cells Determine Preferential Generation of CCR10⁺ NK1.1⁺ ILC1s in Fetal/Neonatal Versus Adult Thymi

(A) Flow cytometric analysis of CD3⁻NK1.1⁺ thymocytes of adult Rag1^−/−CCR10^+/EGFP mice for CCR10(EGFP) and CD127 expression. The dot graph compares the percentages of CCR10⁺ subset of CD3⁻NK1.1⁺ thymocytes in Rag1^−/−CCR10^+/EGFP and CCR10^+/EGFP controls. One dot represents one mouse.

(B) Flow cytometric analysis of CD3⁻NK1.1⁺ cells from the BM of adult Rag1^−/−CCR10^+/EGFP mice for their CCR10(EGFP) and CD127 expression.

(C) Comparison of percentages and numbers of the CCR10⁺CD127⁺ subset of CD3⁻NK1.1⁺ cells in the sLNs and skin of Rag1^−/−CCR10^+/EGFP mice and corresponding CCR10^+/EGFP controls. One dot represents one mouse.

(D) Flow cytometric detection of CD3⁻NK1.1⁺ cells of gated CD45⁺ cells of fetal liver and adult BM donor progenitors in indicated tissues of the same recipient mice analyzed 2 months after transfer.

(E) Comparison of percentages of CD3⁻NK1.1⁺ cells and CD3⁺ T cells of fetal and adult donor-derived CD45⁺ cells in indicated tissues.

One dot represents one mouse. NS, not significant, ∗p < 0.05, ∗∗p < 0.01, ∗∗∗p < 0.001 as determined by two-tailed Student's t test.