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. 2020 Mar 30;23(4):101014. doi: 10.1016/j.isci.2020.101014

Figure 5.

Figure 5

Fetal/Neonatal NK1.1+ ILCs Significantly Contribute to the Establishment of the Skin Immune System

(A) Flow cytometric detection of CD3−NK1.1+ cells in gated CD45+ lymphocytes of the BM, spleen, and skin of CCR10+/EGFP mice treated in utero with anti-NK1.1 or isotype-matched control antibodies. Mice were analyzed 6 weeks after birth.

(B) Comparison of the average percentages of CD3−NK1.1+ cells in BM, spleen, and skin of in utero anti-NK1.1 and control antibody-treated mice. One dot represents one mouse. Data are pooled from two independent experiments as performed in (A).

(C) Comparison of the percentages (left) and numbers (right) of total CD3−NK1.1+ cells in thymi of 2- to 3-day-old WT (+/+) or Vγ43+/− versus Vγ43−/− mice. One dot represents one mouse. Data are combined from two litters.

(D) Comparison of the percentages and numbers of total and CD90.2+ CD3−NK1.1+CD45+CD11b− ILCs in the skin of 10-day-old WT (+/+) or Vγ43+/− versus Vγ43−/− mice.

One dot represents one mouse. NS, not significant, ∗p < 0.05, ∗∗p < 0.01, ∗∗∗p < 0.001 as determined by unpaired two-tailed Student's t test. Error bars denote mean ± SD, and data are combined from five separate litters.