Figure 6.

Skin NK1.1⁺ ILC1s Actively Respond to Bacterial Colonization at Early Postnatal Stages

(A) Comparison of percentages and numbers of total and CD127⁺ CD3⁻NK1.1⁺CD45⁺ lymphocytes of the skin of 10-day-old WT mice raised in SPF versus GF conditions.

(B) Flow cytometric analysis of skin NK1.1⁺ ILCs of 9-day-old WT neonatal mice raised in SPF versus GF conditions for granzyme C (grzmC) expression. NK1.1⁺ ILCs are gated on CD45⁺CD3⁻NK1.1⁺CD90⁺ lymphocytes.

(C) Comparison of average percentages and numbers of grzmC-expressing NK1.1⁺ ILCs in the skin of neonatal mice raised in the SPF versus GF conditions.

One dot represents one mouse. Data are combined from two experiments. ∗p < 0.05, ∗∗p < 0.01 as determined by Student's unpaired two-tailed t test.