Figure 3.

The effect of MPA treatment on MMP-2/-9 mRNA in C6 glial cells. (A) The mRNA levels of MMP-2 and MMP-9 were measured by qRT-PCR in C6 glial cells treated with MPA for 12-h, using MMP-2 and MMP-9 specific primers. (B) At 24-h, the suppression of MMP-9 mRNA levels is no longer detected. Data are represented by the mean ± SEM of three independent qRT-PCR experiments performed in duplicates. The expression levels are represented relative to the GAPDH reference gene. Results are representative of five independent experiments. *p < 0.05 and **p < 0.01.